Premium
Multichannel electrochemical analyses of enzyme reactions
Author(s) -
Kasai Nahoko,
Matsue Tomokazu,
Uchida Isamu
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080808
Subject(s) - horseradish peroxidase , xanthine oxidase , electrochemistry , chemistry , redox , electrode , flow injection analysis , peroxidase , enzyme , inorganic chemistry , nuclear chemistry , combinatorial chemistry , chromatography , organic chemistry , detection limit
Horseradish peroxidase (HRP) and xanthine oxidase (XOD) reactions were investigated using a multichannel electrochemical detector with 16 independent band electrodes held at different potentials. Although the enzyme reaction media gave complicated electrochemical responses in flow injection analysis, responses for a redox species can be extracted easily from the multichannel responses. The course analysis over time of the extracted responses enabled the Michaelis constants and molecular activities of HRP for various electron donors to be established. The electrochemical responses of O 2 −on the μM order in aqueous solution were also washed out from the responses in the XOD reaction media.