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Determination of oxidase enzyme substrates using cross‐flow thin‐layer amperometry
Author(s) -
Yang Liu,
Kissinger Peter T.
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080803
Subject(s) - choline oxidase , glucose oxidase , amperometry , chemistry , immobilized enzyme , chromatography , electrode , biosensor , peroxidase , electrochemistry , biochemistry , enzyme , acetylcholinesterase
Thin‐layer cross‐flow amperometric detectors with enzyme modified working electrodes were developed for the determination of oxidase substrates in flowing streams. The enzyme was covalently immobilized in an osmium redox polymer film on the electrode surface. Electrodes modified with glucose oxidase or lactate oxidase were prepared for the continuous monitoring of glucose or lactate. A peroxidase electrode coupled with oxidase enzyme reactors was also explored for the determination of glucose, lactate, and acetylcholine/choline in continuous monitoring systems or following liquid chromatography. The effects of interferences, oxygen, mass transfer limiting membrane, and perfusate composition on electrode performance are discussed.