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Local anesthetics measured by lipophilic β‐cyclodextrin‐based ion‐selective electrodes
Author(s) -
Kataky Ritu,
Palmer Simon
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080616
Subject(s) - chemistry , bovine serum albumin , chromatography , procaine , potassium , sodium , adamantane , membrane , nuclear chemistry , organic chemistry , biochemistry , pharmacology , medicine
2,3,6‐Trioctyl and 2,6‐didodecyl‐β‐cyclodextrins have been incorporated in ion‐selective electrodes as neutral ionophores for the detection of the local anesthetics procaine, prilocaine, lidocaine and bupivacaine hydrochlorides. Nernstian response and excellent limits of detection were observed (−log [C]≥5.0). Interference from ‘serum’ levels of sodium, potassium and calcium (150.0 mmol dm −3 Na + , 1.26 mmol dm −3 Ca 2+ 4.30 mmol dm −3 K + ), was negligible (−log K i j pot ,) (overall)≥4.0). The major organic interferents were molecules of similar size and structure which caused reduced slope and drift in baseline potentials at 10 mmol dm −3 levels of concentrations. However, the interferences were substantially reduced when the interferent concentration was reduced by a factor of 10. Nernstian response was maintained in the presence of bovine serum albumin (BSA), serum (HS) and aI acid glycoprotein (AAG). A polyurethane, (Tecoflex SG80) membrane matrix was more biocompatible when compared to PVC as evidenced by lower E 0 (internal) shifts with the former.