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Evaluation of a new disposable screen‐printed sensor strip for the measurement of NADH and its modification to produce a lactate biosensor employing microliter volumes
Author(s) -
Sprules Steven D.,
Hart John P.,
Pittson Robin,
Wring Stephen A.
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080608
Subject(s) - biosensor , amperometry , electrode , linear range , lactate dehydrogenase , substrate (aquarium) , chemistry , reference electrode , chromatography , analytical chemistry (journal) , dehydrogenase , phosphate buffered saline , buffer solution , materials science , detection limit , enzyme , biochemistry , electrochemistry , geology , oceanography
A combined sensor strip was fabricated by screen‐printing a Meldola Blue modified carbon working electrode and a Ag/AgCl combined reference/counter electrode alongside each other on a PVC substrate. This was investigated as an amperometric sensor for NADH, at an applied potential of 0 V, both in bulk solutions under hydrodynamic conditions and using 20 μL volumes without agitation. The former technique gave linear responses in the range 3–60 × 10 −6 mol dm −3 and the latter in the range 10−5 × 10 −4 mol dm −3 of NADH. Further modification of the electrodes with NAD + and lactate dehydrogenase converted the device to a biosensor for lactate both in spiked phosphate buffer and bovine serum. The sensors demonstrated good linear responses in the range 0–20 × 10 −3 mol dm −3 .