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Determination of traces of RNA in submicrogram amounts of single‐ or double‐stranded DNAs by means of nucleic acid‐modified electrodes
Author(s) -
Fojta Miroslav,
Doftlková Renata,
Palečhk Emil
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080504
Subject(s) - nucleic acid , rna , dna , chemistry , rnase p , nucleic acid thermodynamics , electrode , rnase h , nucleic acid quantitation , microbiology and biotechnology , biochemistry , chromatography , biology , gene
Recently we have utilized the advantages of nucleic acid‐modified mercury electrodes to develop a method capable of determining RNA traces in samples of double‐stranded DNA [1]. It was, however, impossible to determine less than 10 % of RNA in a single‐stranded DNA sample. Here we show that the DNA voltammetric signals can be selectively suppressed by precipitation with [Co(NH 3 ) 6 ] 3 + while the signal of any RNA digested with RNase A is not influenced. Following from our studies we proposed a voltammetric method for determination of less than 1% of RNA in microgram samples of single‐ and double‐stranded DNA.