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Bienzyme sensors based on poly(anilinomethylferrocene)‐modified electrodes
Author(s) -
Mulchandani Ashok,
Wang ChiaLin
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080503
Subject(s) - chemistry , amperometry , ascorbic acid , horseradish peroxidase , hydrogen peroxide , glucose oxidase , lactic acid , electrode , glassy carbon , peroxidase , biosensor , inorganic chemistry , chromatography , nuclear chemistry , organic chemistry , electrochemistry , biochemistry , enzyme , cyclic voltammetry , food science , biology , bacteria , genetics
Amperometric bienzyme electrodes for glucose, lactic acid and glutamic acid were constructed using glucose oxidase, lactate oxidase and glutamate oxidase immobilized on a horseradish peroxidase‐poly(anilinomethylferrocene)‐modified glassy carbon electrode. In these bienzyme sensors hydrogen peroxide formed by the enzymatic reaction of glucose, lactic acid and glutamic acid with oxygen in presence of the appropriate enzyme was detected at −50 mV (vs. Ag/AgCl), by the horseradish peroxidase‐poly(anilinomethy1ferrocene)‐modified glassy carbon electrode. Because of the detection at a low applied potential, electroactive compounds such as ascorbic acid, uric acid and acetaminophen, did not interfere in analyses. The results of characterization studies of these bienzyme electrodes in terms of their, sensitivity, selectivity, linear detection range, precision, accuracy, operational lifetime stability, and long term storage stability are presented.