Premium
Microdialysis sampling and high performance liquid chromatography with amperometric detection for sugar analysis in milk products
Author(s) -
Mannino Saverio,
Cosio Maria S.,
Zimei Paola
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080410
Subject(s) - amperometry , microdialysis , chromatography , lactose , chemistry , sugar , fructose , high performance liquid chromatography , sucrose , sample preparation , food science , electrode , biochemistry , extracellular , electrochemistry
A rapid and highly efficient method for the direct quantification of the major carbohydrates, glucose, fructose, sucrose and lactose in milk‐based products is described. Rapid and selective monitoring of each sugar is accomplished by microdialysis sampling coupled with HPLC separation and amperometric detection with a chemically modified electrode. This new scheme offers simultaneous monitoring of sugars with little or no sample pretreatment. The results obtained from the application of the proposed procedure to milk and milk‐based products are discussed.