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Enzyme electrode for the determination of bilirubin
Author(s) -
Fortuney Alain,
Guilbault George G.
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080306
Subject(s) - bilirubin oxidase , bilirubin , hydrogen peroxide , chemistry , biliverdin , detection limit , enzyme , platinum , electrode , inorganic chemistry , nuclear chemistry , chromatography , biochemistry , heme , catalysis , biology , heme oxygenase , endocrinology
Bilirubin oxidase (EC 1.3.3.5) catalyzes the oxidation of bilirubin to biliverdin with the concurrent reduction of oxygen to hydrogen peroxide. A platinum electrode was prepared with an immobilized bilirubin oxidase membrane. Bilirubin was determined by monitoring the increase in hydrogen peroxide concentration from the enzyme reaction. The calibration plot for bilirubin shows linearity up to 300μM. The detection limit is 0.7μM.