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Development and optimization of a solid composite tyrosinase biosensor for phenol detection in flow injection systems
Author(s) -
Mareikelutz E. S.,
Dominguez Elena
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080204
Subject(s) - tyrosinase , biosensor , composite number , phenol , flow injection analysis , central composite design , chemistry , flow (mathematics) , chromatography , combinatorial chemistry , nanotechnology , materials science , detection limit , organic chemistry , composite material , response surface methodology , mathematics , enzyme , geometry
Bulk‐modified epoxy‐graphite tyrosinase biosensors were fabricated by four different procedures. The influence of these fabrication procedures on the analytical performance of the enzyme electrode in an amperometric wall‐jet flow cell has been studied. The bioprobe performance is assessed by cyclic voltammetry. Higher current densities and narrower peaks were obtained when the enzyme was introduced in the dry state into the epoxy‐graphite material, instead of introducing it previously dissolved in the buffer. In the F1 system responses of 11.79 μA cm −2 and 1.43 μA cm −2 are then obtained for catechol and phenol respectively for 50 μL injections of 20 μM solutions. Moreover, if gold/palladium is introduced into the epoxy‐graphite, a further increase in current is achieved resulting in 27.70μA cm −2 and 4.90μA cm −2 for catechol and phenol, respectively. This biosensor can operate in aqueous as well as in mixed aqueous‐organic environments.