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Biosensors based on cross‐linking of biotinylated glucose oxidase by avidin
Author(s) -
Vreeke Mark S.,
Rocca Patrick
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080112
Subject(s) - glucose oxidase , avidin , horseradish peroxidase , biotinylation , biosensor , chemistry , redox , electrode , linear range , peroxidase , amperometry , nuclear chemistry , combinatorial chemistry , inorganic chemistry , electrochemistry , chromatography , biochemistry , detection limit , enzyme
Avidin, having four biotin binding units, cross‐links and immobilizes glucose oxidase (GOX) labeled with multiple biotins (B‐GOX) when solutions of B‐GOX and avidin are mixed on an electrode. The H 2 O 2 flux generated in the B‐GOX catalyzed oxidation of glucose by dissolved O 2 is measured as an electroreduction current at a horseradish peroxidase (HRP) redox conducting hydrogel electrode poised at +100 mV (Ag/AgCl). The sensitivity of the resulting glucose sensor is 0.14 A cm −2 M −1 with a linear range up to 2mM glucose.