Biosensors based on cross‐linking of biotinylated glucose oxidase by avidin | Zendy

Vreeke Mark S. | Zendy; Rocca Patrick | Zendy

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Biosensors based on cross‐linking of biotinylated glucose oxidase by avidin

Author(s) -

Vreeke Mark S.,

Rocca Patrick

Publication year - 1996

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.1140080112

Subject(s) - glucose oxidase , avidin , horseradish peroxidase , biotinylation , biosensor , chemistry , redox , electrode , linear range , peroxidase , amperometry , nuclear chemistry , combinatorial chemistry , inorganic chemistry , electrochemistry , chromatography , biochemistry , detection limit , enzyme

Avidin, having four biotin binding units, cross‐links and immobilizes glucose oxidase (GOX) labeled with multiple biotins (B‐GOX) when solutions of B‐GOX and avidin are mixed on an electrode. The H 2 O 2 flux generated in the B‐GOX catalyzed oxidation of glucose by dissolved O 2 is measured as an electroreduction current at a horseradish peroxidase (HRP) redox conducting hydrogel electrode poised at +100 mV (Ag/AgCl). The sensitivity of the resulting glucose sensor is 0.14 A cm −2 M −1 with a linear range up to 2mM glucose.

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