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Adsorptive stripping potentiometry of DNA at electrochemically pretreated carbon paste electrodes
Author(s) -
Wang Joseph,
Cai Xiaohua,
Jonsson Colleen,
Balakrishnan Mini
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080105
Subject(s) - stripping (fiber) , potentiometric titration , electrode , carbon paste electrode , electrochemistry , chemistry , carbon fibers , anodic stripping voltammetry , chromatography , materials science , cyclic voltammetry , composite number , composite material
Potentiometric stripping analysis is employed for highly sensitive measurements of single‐stranded DNA at carbon paste electrodes. Electrochemical (oxidative) pretreatment is used to condition the carbon paste surface prior to the accumulation process. The low background response of the potentiometric stripping mode offers convenient quantitation of low nanogram quantities of DNA. Experimental variables of the pretreatment and measurement procedures are explored and optimized. Results are also reported for the quantitation of nanogram levels of the long terminal repeat (LTR) from the human leukemia virus and for the stripping operation of microfabricated screen‐printed electrodes.