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Variation in PED response at a gold microelectrode as a function of waveform parameters when applied to alditols and carbohydrates separated by capillary electrophoresis
Author(s) -
Roberts Robert E.,
Johnson Dennis C.
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140071104
Subject(s) - capillary electrophoresis , electrode , detection limit , analytical chemistry (journal) , waveform , chemistry , microelectrode , chromatography , electrochemistry , capillary action , monosaccharide , noise (video) , materials science , voltage , organic chemistry , physics , quantum mechanics , composite material , image (mathematics) , artificial intelligence , computer science
Abstract Results are presented from a systematic study of the noise ( N ) and peak‐to‐noise ratio ( P / N ) obtained for pulsed electrochemical detection (PED) at a gold micro‐wire electrode applied to a mixture of alditols and monosaccharides separated by capillary electrophoresis (CE). The electrode potentials and time periods for oxidative cleaning and reductive reactivation of the electrode were held constant in the PED waveform at minimum allowable values and the time period for digital integration of electrode current ( t int ) was varied from 50 to 800 ms by 50‐ms intervals. The value of N was virtually independent of t int and P / N increased approximately as a linear function of t int in the range 50–200 ms corresponding to waveform frequencies in the range 4.5–2.7 Hz. A detection limit of 9 fmol glucose was determined for a 4‐nL injection using t int = 200 ms.