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Redox polymers for electrocatalytic oxidation of NADH – A random block methyl‐siloxane polymer containing meldola blue
Author(s) -
Persson Björn,
Lee Hung Sui,
Gorton Lo,
Skotheim Terje,
Bartlett Philip
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140071005
Subject(s) - phenoxazine , redox , polymer , chemistry , nicotinamide adenine dinucleotide , siloxane , electrocatalyst , electrode , kinetics , inorganic chemistry , polymer chemistry , electrochemistry , nuclear chemistry , organic chemistry , nad+ kinase , medicine , physics , phenothiazine , quantum mechanics , pharmacology , enzyme
Abstract A water‐insoluble modified methyl‐siloxane polymer which held 9‐dimethylaminobenzo[a]phenoxazine‐7‐ium (Meldola Blue) units, was synthesized, characterized, and used to drop‐coat solid graphite electrodes. The electrocatalytic oxidation of the nicotinamide adenine dinucleotide (NADH) in the polymer could be described by the Michaelis–Menten type of kinetics as seen from steady‐state measurements in 0.25 M phosphate buffer, pH7, at 0 mV (vs. SCE). The electrocatalytic properties of the redox polymer was evaluated using the film thickness as a major variable. The theoretical model found to describe the NADH oxidation at the redox polymer modified electrode, is presented. A maximum NADH sensitivity of 40 μA cm −2 mM −1 at a coverage of 0.5 μg cm −2 was indicated.