Premium
Kinetic characterization of the effects of organic solvents on the performance of a peroxidase‐modified electrode in detecting peroxides, thiourea and ethylenethiourea
Author(s) -
Adeyoju Olubunmi,
Iwuoha Emmanuel I.,
Smyth Malcolm R.
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140071003
Subject(s) - thiourea , chemistry , horseradish peroxidase , biosensor , acetonitrile , solvent , peroxidase , hydrogen peroxide , substrate (aquarium) , electrode , nuclear chemistry , chromatography , inorganic chemistry , organic chemistry , enzyme , biochemistry , oceanography , geology
Horseradish peroxidase (HRP) modified electrodes were constructed by entrapping the enzyme in an Eastman AQ 55D polymer. The biosensor detects butanone peroxide (BTP) as a substrate, and thiourea (THU) and ethylenethiourea (ETU) as HRP inhibitors. The kinetic parameters for this sensor in organic media such as I max and K ′ m for BTP detection and K ′ i for ETU and THU detection were evaluated. The differences in the values of these parameters were explained in terms of the physico‐chemical properties of the organic reaction media. A possible solvent‐induced deviation of the biosensor behavior from Michaelis–Menten kinetics in the detection of ETU and THU was evaluated using the Hill analysis. The least deviation was observed in acetonitrile. Spectroelectrochemistry was also used to qualitatively assess solvent–HRP, BTP–HRP and inhibitor–HRP interactions.