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Chemically amplified detection of catecholamines by liquid chromatography using an L ‐ascorbic acid carrier
Author(s) -
Uchiyama Shunichi,
Itoi Naoyuki,
Husehe Yusushi
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070807
Subject(s) - ascorbic acid , chemistry , tyrosinase , detection limit , chromatography , dopamine , substrate (aquarium) , norepinephrine , biochemistry , enzyme , biology , ecology , food science , neuroscience
Abstract The oxygen consumed during the tyrosinase reaction is significantly amplified by L ‐ascorbic acid due to a substrate recycling process; the substrates have to be separated in order to obtain individual amplified current responses of each substrate. In this work, the chemically amplified current responses of catecholamines were obtained individually by liquid chromatography using an immobilized tyrosinase reactor and an L ‐ascorbic acid carrier solution. The highly sensitive determinations of L ‐dopa, dopamine, and norepinephrine were carried out individually using a Clark type oxygen electrode detector. The amplification factors of 1 × 10 −7 M L ‐dopa, norepinephrine, and dopamine were found to be 224, 357, and 568, respectively, when 5 × 10 3 M L ‐ascorbic acid solution was used as a carrier; the detection limit for dopamine was found to be 5 × 10 −9 M.