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Biosensing of tyrosinase inhibitors in nonaqueous solvents
Author(s) -
Stancik Lubor,
Macholán Lumír,
Scheller Frieder
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070709
Subject(s) - tyrosinase , biosensor , thiourea , chemistry , amperometry , phenol , dialysis tubing , electrode , chromatography , detection limit , combinatorial chemistry , membrane , enzyme , organic chemistry , electrochemistry , biochemistry
An amperometric biosensor based on a Clark oxygen electrode coupled with a biocatalytic layer containing tyrosinase has been developed for measurement in nonaqueous solvents. Glass filter paper was soaked with the enzyme and fixed on top of the electrode by a dialysis membrane. Thiourea derivatives were detected in hexane by evaluating the decrease in the response towards phenol. This method was fast and highly sensitive with detection limits in the range of 13–181 nM depending on the structure of the inhibitor compounds. The response time was up to 10min and depended on the inhibitor's structure. The mechanism of inhibition is discussed using kinetic parameters.