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Amperometric glucose biosensors based on an osmium (2 + /3 + ) redox polymer‐mediated electron transfer at carbon paste electrodes
Author(s) -
Pravda Miloslav,
Adeyoju Olubunmi,
Iwuoha Emmanuel I.,
Vos Johannes G.,
Smyth Malcolm R.,
Vytras Karel
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070704
Subject(s) - osmium , amperometry , redox , electron transfer , biosensor , chemistry , electrode , electrochemistry , polymer , inorganic chemistry , carbon fibers , analytical chemistry (journal) , nuclear chemistry , photochemistry , materials science , chromatography , organic chemistry , ruthenium , biochemistry , catalysis , composite material , composite number
Abstract Four glucose sensors have been developed by modifying carbon paste electrodes with glucose oxidase as a biocomponent and a conducting redox osmium‐polymer as the mediating compound. The enzyme was immobilized by crosslinking with glutaraldehyde and bovine serum albumin in three modifications of electrodes and physical adsorption in one electrode. The optimal concentrations of the modifying compounds were determined and the response of the sensors to 2–20 mM glucose were characterized. The influence of pH, temperature, interferences, and storage on the responses of the sensors were investigated.