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Acetylcholinesterase‐based amperometric sensor
Author(s) -
Stoytcheva Margarita
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070610
Subject(s) - amperometry , acetylthiocholine , acetylcholinesterase , reproducibility , chemistry , repeatability , electrode , analytical chemistry (journal) , materials science , chromatography , electrochemistry , organic chemistry , enzyme , aché
An amperometric sensor has been created using the hydrolysis of acetylthiocholine iodide, which is catalyzed by the acetylcholinesterase enzyme and generates an electroactive product. An electrochemically modified graphite electrode is used, with acetylcholinesterase covalently bonded directly to its surface. The characteristics of the sensor, such as the response time, repeatability, reproducibility, stability, dynamic range, activation energy, and temperature coefficient of the current are evaluated. The optimum working conditions (pH, temperature, and rotation speed) are established.