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Amperometric gas‐diffusion/enzyme electrode
Author(s) -
Iliev Ilia,
Kaisheva Anastassia,
Scheller Frieder,
Pfeiffer Dorothea
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070607
Subject(s) - amperometry , glucose oxidase , clark electrode , electrode , gas diffusion electrode , chemistry , electrolyte , catalysis , immobilized enzyme , biosensor , diffusion , gaseous diffusion , inorganic chemistry , oxygen , electrochemistry , analytical chemistry (journal) , chromatography , organic chemistry , enzyme , biochemistry , physics , thermodynamics
An amperometric enzyme gas‐diffusion electrode has been developed which combines a porous, air breathing gas‐diffusion electrode with an enzyme [glucose oxidase (GOD)] immobilized with Nation into its porous catalytic layer. The catalytic layer contains pyrolyzed Co‐ tetramethoxyphenylporphyrine (CoTMPP), which is very active towards the electrochemical oxidation of H 2 O 2 . The behavior of this electrode was investigated when the oxygen needed for the enzymatic reaction was supplied from the air (in the gas phase) through the porous structure of the gas‐diffusion electrode. It was shown that in the presence of the substrate (glucose), the amperometric signal obtained in this mode of operation was much higher than that obtained when only the oxygen dissolved in the electrolyte was involved in the enzymatic reaction. The developed enzyme gas‐diffusion electrode allows a versatile and rapid change in the O 2 concentration in the zone where the enzymatic reaction is taking place. This electrode can be used as a model system for the investigation of the influence of O 2 on the performance of both the peroxide or the mediated enzyme electrodes in an effort to develop oxygen independent biosensors.