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Amplified biosensing of alcohol based on biocatalytic accumulation of the mediator
Author(s) -
Wang Joseph,
Naser Najih
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070411
Subject(s) - biosensor , alcohol dehydrogenase , mediator , alcohol , chemistry , cofactor , nicotinamide , wine , chromatography , combinatorial chemistry , biochemistry , enzyme , biology , endocrinology , food science
A novel strategy for enhancing the sensitivity of alcohol‐dehydrogenase amperometric biosensors, based on biocatalytic accumulation of the mediator, is described. By performing the biochemical/chemical step under open‐circuit conditions, it is possible to accumulate the reduced form of the Meldola blue mediator, prior to the chronoamperometric quantitation of the enriched layer. Such temporal separation of the recognition and transduction events offers a 35‐fold sensitivity enhancement (following a 5‐min accumulation). Factors influencing the response are explored and applicability to various wine samples is illustrated. The new amplification strategy can be extended to biosensors based on other nicotinamide‐cofactor dependent dehydrogenases.