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Tungsten sensor for amperometric detection of organic thiols and proteins
Author(s) -
Alexander Peter W.,
Hidayat Achmad,
Hibbert D. Brynn
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070318
Subject(s) - amperometry , cysteine , chemistry , thiourea , mercury (programming language) , glutathione , thiol , tungsten , inorganic chemistry , inert , detection limit , electrode , chromatography , electrochemistry , organic chemistry , enzyme , computer science , programming language
An amperometric sensor constructed with a metallic tungsten wire is reported for the indirect detection of organic thiols and proteins. Cysteine added to mercury( II ) (40.0μM) buffered in 5.0 mM sodium acetate at pH 4.7 causes a decrease in the reduction current of mercury at −0.2 V (vs. SCE). Detection limits were found to be in the range 0.05–0.2 μg/mL for cysteine, thiourea and glutathione, but were poorer for albumin and DNA, showing this method is relatively selective for cysteine. The advantages of the tungsten electrode are its low cost, miniature size, and its inert oxide surface which does not amalgamate with mercury.