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Determination of aminopyrine and its metabolite in biological fluid by liquid chromatography/electrochemistry with a glassy carbon electrode dispersed with α‐alumina particles
Author(s) -
Li Huimei,
Wang Erkang
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070315
Subject(s) - glassy carbon , electrochemistry , electrode , carbon fibers , metabolite , chromatography , biological fluids , chemistry , materials science , cyclic voltammetry , biochemistry , composite material , composite number
A sensitive high‐performance liquid chromatographic method has been developed for the quantitative determination of aminopyrine (AM) and its metabolite 4‐aminoantipyrine (AAN). The method utilizes reverse‐phase chromatography/amperometric detection with a glassy carbon electrode dispersed with α‐alumina particles as the working electrode, on which the oxidation of AM and AAN was greatly improved compared with that on a bare glassy carbon electrode. As a result, the detection limit was as low as 1.4 ng for AM and 0.8 ng for AAN, and the calibration plots for the above compounds have wide linear ranges from 100 μg/mL to 100 μg/mL and 60 ng/mL to 80 μg/mL (for AM and AAN, respectively). The above method was applied for the detection of these materials in human urine with satisfactory results.