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Application of dual‐pulse staircase voltammetry for simultaneous determination of glucose and fructose
Author(s) -
Fung Y.S.,
Mo S.Y.
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070212
Subject(s) - fructose , chemistry , chromatography , voltammetry , analytical chemistry (journal) , cyclic voltammetry , electrochemistry , electrode , food science
A new electroanalytical method, DPSV, employing dual pulses (0.7V and −0.8V) prior to anodic scan (using staircase voltammetry) is developed for simultaneous determination of glucose and fructose. The electrochemistry of both sugars was studied prior to optimizing the operational parameters. The linear ranges were found to be 0.01–2.0 m M for both sugars, precisions (mean = 0.10 m M and n = 8) 1.8% and 2.4% and detection limits (estimated by 3 times the standard deviation) 0.001 and 0.003 m M for glucose and fructose, respectively. To establish the reliability of the method, various food additives were tested and found to give no interference effect under normal concentrations. HPLC method was carried out in parallel with the method for analyzing food, drink and honey samples. No statistically significant difference in the results was obtained for methods.