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Simultaneous assays of glucose, urate, and cholesterol in blood serum by amperometric flow injection analysis
Author(s) -
Yao T.,
Satomura M.,
Nakahara T.
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070209
Subject(s) - flow injection analysis , chromatography , chemistry , amperometry , nafion , uric acid , hydrogen peroxide , biosensor , platinum , cellulose acetate , coefficient of variation , electrode , cellulose , biochemistry , detection limit , electrochemistry , catalysis
Glucose, uric acid, and cholesterol in serum were assayed simultaneously in a flow injection system by using a 16‐way switching valve and a parallel configuration of three immobilized enzyme reactors. Nafion film‐coated and Nafion/cellulose acetate film‐coated platinum electrodes were used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interferences from electroactive species such as L ‐ascorbate and proteins present in the serum. The signal current was linearly related to the glucose concentration in the range of 5–700 mg/dL, to the urate concentration in the range of 1–80 mg/dL, and to the cholesterol concentration in the range of 5–100 mg/dL. The precision (coefficient of variation) was better than 2.8% for the simultaneous assay of the three species in human control serum. The assay speed was up to 32 samples per hour.