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Dual enzyme amperometric biosensor for putrescine with interference suppression
Author(s) -
Yang X.,
Rechnitz G. A.
Publication year - 1995
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140070202
Subject(s) - putrescine , cadaverine , biosensor , chemistry , detection limit , chromatography , amperometry , spermidine , glutaraldehyde , electrode , nuclear chemistry , biochemistry , enzyme , electrochemistry
A putrescine biosensor has been developed. Putrescine oxidase, peroxidase, and bovine serum albumin were cross‐linked directly on the surface of a graphite electrode by glutaraldehyde. The sensor was used to determine putrescine at 0 mV (vs. Ag/AgCl reference electrode) in a potassium phosphate buffer (pH 7.4) solution containing 4 mM dopamine (3‐hydroxytyramine). The response of putrescine was linear up to 600 μ M with a standard deviation of 2.8%. The detection limit was 5 μ M . The response time was around 30 s. The lifetime was over 30 days when the electrode was used every day. The interferences of cadaverine and spermidine could be eliminated by added dopamine which, apparently, inhibits the enzymatic conversion of these substrates more strongly than that of putrescine.