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Evaluation of mediators for development of amperometric microbial bioelectrodes
Author(s) -
Kaláb TomáŠ,
Skládal Petr
Publication year - 1994
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140061114
Subject(s) - paracoccus denitrificans , amperometry , chemistry , ferrocene , cyanide , membrane , biosensor , bacteria , vesicle , chromatography , biochemistry , combinatorial chemistry , electrode , electrochemistry , inorganic chemistry , biology , enzyme , genetics
The microbial biosensors were constructed from carbon paste electrodes (CPEs) modified with insoluble mediators and immobilized membrane vesicles or whole cells of the bacterium Paracoccus denitrificans . The best results were obtained with membrane vesicles and ferrocene (FC). This system could serve for determination of succinate, D‐lactate, and L‐lactate. Significant increase of signal was obtained when competitive flux of electrons to oxygen was blocked by inhibiting terminal oxidases using cyanide.