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Lactate biosensor based on a lactate dehydrogenase/nicotinamide adenine dinucleotide biocomposite
Author(s) -
Wang Joseph,
Chen Qiang
Publication year - 1994
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140061007
Subject(s) - biocomposite , biosensor , nicotinamide adenine dinucleotide , lactate dehydrogenase , detection limit , chemistry , nad+ kinase , amperometry , chromatography , biochemistry , materials science , electrode , enzyme , electrochemistry , composite number , composite material
A new biocomposite, fabricated by incorporating lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide (NAD + ) within a graphite‐epoxy matrix, is used as a polishable (renewable) and robust biosensor for lactate. Highly sensitive and rapid amperometric biosensing is accomplished at relatively low potentials. The regeneration capability of the biocomposite probe circumvents problems of surface fouling inherent to the detection of NADH. Flow injection detection of lactate, with a sample throughput of 60/h, detection limit of 5 × 10 −4 M, precision of 2.5% (RSD for n = 40), and linearity up to 2 × 10 −2 M, is illustrated.