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Improved performance of carbon paste amperometric biosensors through the incorporation of fumed silica
Author(s) -
Wang Joseph,
Naser Najih
Publication year - 1994
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140060707
Subject(s) - fumed silica , ascorbic acid , amperometry , biosensor , ferrocene , chemistry , carbon fibers , chemical engineering , immobilized enzyme , matrix (chemical analysis) , inorganic chemistry , organic chemistry , electrochemistry , materials science , chromatography , electrode , enzyme , biochemistry , food science , composite material , composite number , engineering
The sensitivity and stability of carbon paste enzyme elearodes are greatly enhanced through the incorporation of fumed silica within the paste matrix. The improved performance is attributed to the adsorptive and electrocatalytic properties of the silica nanoparticles. The electrocatalytic action results in a substantial lowering of the overvoltage for both the oxidation and reduction of the enzymatically liberated peroxide species. Highly selective, low‐potential, biosensing of glucose is thus accomplished in the presence of coexisting acetaminophen, ascorbic acid, or uric acid. The enhanced performance accrued from the presence of silica is also illustrated for mediator (dimethyl ferrocene)‐containing pastes. High enzymatic activity is maintained over prolonged periods. The silica modification strategy provides an effective, fast, and inexpensive route for immobilizing enzymes within carbon paste matrices.