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Amperometric biosensors for detection of L‐ and D‐amino acids based on coimmobilized peroxidase and L‐ and D‐amino acid oxidases in carbon paste electrodes
Author(s) -
Kacaniklic V.,
Johansson K.,
Marko–Varga G.,
Gorton L.,
Jönsson–Pettersson G.,
Csöregi E.
Publication year - 1994
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140060505
Subject(s) - amperometry , hydrogen peroxide , biosensor , peroxidase , chemistry , immobilized enzyme , glucose oxidase , horseradish peroxidase , amino acid , flow injection analysis , carbon paste electrode , electrode , nuclear chemistry , inorganic chemistry , detection limit , chromatography , electrochemistry , organic chemistry , biochemistry , enzyme , cyclic voltammetry
An apparent direct electron transfer between various electrode materials and peroxidases immobilized on the surface of the electrode has been reported in the last few years. An electrocatalytic reduction of hydrogen peroxide promoted by the immobilized peroxidase starts at about +600 mV (vs. Ag/AgCl) at neutral pH. The efficiency of the electrocatalytic current increases as the applied potential is made more negative and starts to level off it about −100 mV (vs. Ag/AgCl). Amperometric biosensors for hydrogen peroxide can be constructed with these kinds of peroxidase modified electrodes. By coimmobilizing a hydrogen peroxide‐producing oxidase with the peroxidase, amperometric biosensors can be made responding to the substrate of the oxidase within a potential range essentially free of interfering electrochemical reactions. Sensors for L‐ and D‐amino acids are shown based on coimmobilizing HRP with L‐amino acid oxidase (L‐AAOD) or D‐amino acid oxidase (D‐AAOD). The effects of the immobilization procedure, buffer (flow carrier), pH, taken amounts of enzymes, flow rate, and injection volume on the response were investigated. The addition of polyethylenimine (PEI) into the paste was found to increase the response considerably. All 20 of the most common L‐amino acids could be detected.