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Simultaneous determination of glucose and sucrose by a dual‐working electrode multienzyme sensor flow‐injection system
Author(s) -
Zhang Xianen,
Rechnitz Garry A.
Publication year - 1994
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140060503
Subject(s) - invertase , sucrose , glucose oxidase , mutarotation , chemistry , chromatography , membrane , immobilized enzyme , analytical chemistry (journal) , biosensor , nuclear chemistry , enzyme , biochemistry , stereochemistry
Abstract A dual‐working electrode (K 1 and K 2 ) multienzyme sensor, made of carbon paste, glucose oxidase (GOD) membrane (on K 1 ) and invertase (INV)/mutarotase (MUT)/GOD membrane (on K 2 ), is proposed for simultaneous determination of glucose and sucrose. Interference to the K 2 caused by H 2 O 2 produced in the enzyme reaction on K 1 can be eliminated by depositing a catalase (CAT) layer between K 1 and K 2 . Acceleration of glucose mutarotation by MUT was found to be three times faster than by phosphate buffer. Glucose was determined directly from the response of K 1 , while sucrose was computed based on a formula derived from a two‐point calibration method. The maximum concentration of sucrose that could be determined depended strongly on the concentration of coexisting glucose. The activities of immobilized GOD, INV, MUT, and CAT were examined individually and found stable at room temperature for at least 1 month. The final loss of sucrose response activity was found to be due to denaturation of INV.