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Amperometric detection of purine nucleotides utilizing immobilized enzyme reactors and on‐line amplification by substrate recycling
Author(s) -
Yao Toshio,
Tsureyama Kiminori
Publication year - 1994
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140060215
Subject(s) - purine nucleoside phosphorylase , substrate (aquarium) , chemistry , detection limit , nucleotide , chromatography , amperometry , immobilized enzyme , purine , flow injection analysis , alkaline phosphatase , xanthine , xanthine oxidase , biochemistry , enzyme , biology , electrode , ecology , gene , electrochemistry
Flow‐injection systems with an immobilized enzyme reactor are proposed for the amperometric detection of purine nucleotides. One system studied is based on the combined use of an alkaline phosphatase (AlP) immobilized reactor and a purine nucleoside phosphorylase (PNP)‐xanthine oxidase (XO) coimmobilized reactor, which responds to purine nucleotides With high selectivity. Another system with a coimmobilized AlP‐PNP‐XO reactor gives responses amplified by substrate recycling. When carrier solution was pumped at a flow rate of 0.5 mL min −1 , purine nucleotides could be detected with 9 to 26 times the sensitivity in the latter system compared with the former, with the detection limit of 4 × 10 −8 M for a 20 μL injection.