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Postchromatographic electrochemical detection of carbohydrates at a silver oxide electrode
Author(s) -
Tougas Terrence P.,
Debenedetto Mark J.,
Demott James M.
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050808
Subject(s) - oxide , electrochemistry , electrode , chemistry , inorganic chemistry , silver oxide , high performance liquid chromatography , chromatography , electrochemical cell , materials science , chemical engineering , organic chemistry , engineering
Abstract Silver electrodes, when conditioned at sufficiently positive potentials in the presence of a strong base, can be made to form a silver(I) oxide or silver(II) oxide surface. In the presence of this oxide surface, carbohydrates may be oxidized and quantitated by means of an electrocatalytic scheme. The current which flows to reoxidize the charge‐transfer mediator (silver oxide) is directly related to concentration of carbohydrate. Rectilinear response is obtained over at least two decades of concentration (10 4 M to 10 −6 M). Design of a prototype electrochemical cell and studies of optimum cell conditions are discussed. The application of a high‐performance liquid chromatography (HPLC) detector for the separation of some common sugars is illustrated.