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Controlled electrochemical preparation of enzymatic layers for the design of amperometric biosensors
Author(s) -
CocheGuerente Liliane,
Cosnier Serge,
Innocent Christophe,
Mailley Pascal,
Moutet JeanClaude,
Morélis Renée M.,
Leca BÉAtrice,
Coulet Pierre R.
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050804
Subject(s) - choline oxidase , glucose oxidase , amperometry , biosensor , pyrrole , electrode , monomer , chemistry , electrochemistry , platinum , immobilized enzyme , enzyme , combinatorial chemistry , materials science , chemical engineering , chromatography , organic chemistry , polymer , biochemistry , catalysis , acetylcholinesterase , engineering
A newly synthesized pyrrole derivative was used for enzyme entrapment on carbon or platinum electrodes by electropolymerization. Three enzymes were tested: glucose oxidase (GOD), tyrosinase, and choline oxidase. Contrary to other techniques using electropolymerization in solution, a drop of enzyme‐monomer mixture was deposited and allowed to dry on the electrode surface before the electropolymerization. This procedure enables one to control the amount of entrapped enzyme and to easily obtain multilayer assemblies.