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Graphite‐teflon enzyme electrode
Author(s) -
Wang Joseph,
Reviejo A. Julio,
Angnes Lucio
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050708
Subject(s) - graphite , glucose oxidase , electrode , hydrogen peroxide , horseradish peroxidase , biosensor , materials science , fabrication , detection limit , immobilized enzyme , chemical engineering , chemistry , analytical chemistry (journal) , nanotechnology , chromatography , composite material , organic chemistry , enzyme , medicine , alternative medicine , pathology , engineering
Abstract The fabrication and performance of graphite‐Teflon‐enzyme electrodes are described. The new bulk‐modified composite enzyme electrodes couple the advantages of renewable (polishable) surfaces, mechanical rigidity, fast response, and favorable signal‐to‐noise characteristics. The new fabrication strategy is illustrated for the immobilization of horseradish peroxidase and glucose oxidase, in connection with measurements of peroxide species and glucose, respectively. The incorporation of a ferrocene mediator to yield a reagentless device is also illustrated. Flow injection measurements of hydrogen peroxide with the graphite‐Teflon‐peroxidase detector are carried out at 120 samples/hour, to yield a detection limit of 2.5 × 10 −6 M and a relative standard deviation of 2%. Applicability to organic‐phase biosensing is illustrated.