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Nafion‐crown ether modified carbon fiber electrodes: New microbiosensors for detection of neurotransmitters in vitro and in vivo
Author(s) -
Baumeyer Thomas,
Dittrich JÜRgen,
Crespi Francesco
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050706
Subject(s) - nafion , in vivo , crown ether , chemistry , voltammetry , ascorbic acid , cyclic voltammetry , ether , electrode , detection limit , fiber , chromatography , biophysics , nuclear chemistry , electrochemistry , organic chemistry , biology , ion , microbiology and biotechnology , food science
Based on experience in in vivo voltammetry with Nafion carbon fiber electrode coated (CFE), a new kind of modification, was developed based on mixing Nafion and crown ether (dibenzo‐18‐crown‐6) solutions. Nafion‐crown ether electrodes (NA‐CRO‐CFEs) offer higher sensitivity to dopamine than Nafion‐CFEs. The improvement of detection limit is caused by enrichment of the neurotransmitter in polymermembrane by interaction of oxygen atoms of the crown ether and the positively charged amino group of catecholamine. With NA‐CRO‐CFEs one is able to detect dopamine and serotonin in physiological basal concentrations in vitro and in vivo without overlapping of oxidation peaks of ascorbic acid or metabolites. Identification of in vivo peaks is discussed with in vitro and in vivo experiments.