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Platinized carbon ultramicroelectrodes as glucose biosensors
Author(s) -
Reynolds Eugene R.,
Yacynych Alexander M.
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050506
Subject(s) - glucose oxidase , chemistry , biosensor , hydrogen peroxide , carbodiimide , electrode , platinum , electrochemistry , glutaraldehyde , carbon nanotube , carbon fibers , inorganic chemistry , chromatography , nanotechnology , organic chemistry , materials science , catalysis , biochemistry , composite material , composite number
Miniaturized glucose biosensors were constructed by all‐chemical means from disk‐shaped carbon ultramicroelectrodes (UMEs) (diameter = 8 μm). These ultramicrobiosensors (UMBs) are capable of direct measurement of glucose in the presence of interfering species. Carbon UMEs were partially platinized by the electrochemical reduction of a platinum (IV) complex. Platinization of the carbon UMEs gave an increased current response to hydrogen peroxide and allowed the modified electrode to be operated at a lower oxidative potential. Glucose oxidase (E.C. 1.1.3.4.) was immobilized either by glutaraldehyde cross‐linking or by direct covalent attachment to the electrode surface using carbodiimide. Response time (100% response) was 15–45 seconds. Response was linear over the range of 3–7 mM glucose. An electropolymerized film of poly(1,3‐diaminobenzene) was used to prevent interfering species.