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Monoalkylferrocene‐mediated amperometric enzyme electrodes for glucose determination
Author(s) -
Razumas Valdemaras,
Kulys Juozas,
Knichel Martin,
Wiemhöfer HansDieter,
Göpel Wolfgang
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050505
Subject(s) - glucose oxidase , amperometry , biosensor , electrode , chemistry , analytical chemistry (journal) , graphite , chromatography , electrochemistry , biochemistry , organic chemistry
Abstract Amperometric biosensors for the determination of β‐ D ‐glucose are described. Glucose‐sensitive graphite electrodes are prepared by adsorptive immobilization of n ‐butylferrocene ( n ‐BFc), ter ‐butylferrocene ( t ‐BFc), or ethylferrocene (EFc). Glucose oxidase from Aspergillus niger is immobilized on the monoalkylferrocene‐modified surfaces of the graphite electrodes by covalent attachment. The biosensors based on n ‐BFc, t ‐BFc, and EFc could be used at potentials of 0.3, 0.325, and 0.275 V, respectively (vs. SCE reference electrode). A linear steady‐state current response, proportional to the glucose concentration in a range of 1–20 mM, is observed for the t ‐BFc‐ and EFc‐mediated enzyme electrodes. Data are presented on the influence of potential, oxygen, pH, and temperature upon the electrodes. Results are discussed on the basis of the structural features of the mediators.