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Selective biocatalytic measuring cell for flow injection analysis
Author(s) -
Tarcali József,
Nagy Géza,
Pungor Ernö
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050403
Subject(s) - hydrogen peroxide , flow injection analysis , cellulose , electrode , dialysis tubing , cellulose acetate , materials science , platinum , carboxymethyl cellulose , fiber , chemistry , chromatography , analytical chemistry (journal) , chemical engineering , catalysis , detection limit , biochemistry , composite material , organic chemistry , membrane , sodium , engineering
A flow‐through measuring cell developed for flow injection analysis (FIA) measurements is described. The cell works detecting amperometrically the hydrogen peroxide formed in an enzyme‐catalyzed oxidation reaction of different substrates. The electrode consists of a thin, hollow dialysis fiber introduced into the flow analysis channel, an enzyme solution film pumped inside the fiber, and a cellulose acetate protective layer‐coated thin platinum wire‐base sensor housed inside the hollow fiber. The measuring cell for two different model compounds, glucose and ethanol, was well applicable for the concentration determinations in small volume solution aliquots. Important advantages of the cell are its versatility and its easy activation for measurements.