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Cathodic stripping voltammetric measurements of cholesterol following adsorptive preconcentration at the static mercury drop electrode
Author(s) -
Tuzhi Peng,
Huiping Li,
Rongshan Lu
Publication year - 1993
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140050214
Subject(s) - hanging mercury drop electrode , mercury (programming language) , drop (telecommunication) , cathodic stripping voltammetry , electrode , cathodic protection , dropping mercury electrode , chemistry , analytical chemistry (journal) , materials science , chromatography , voltammetry , inorganic chemistry , electrochemistry , telecommunications , computer science , programming language
A new electrochemical method for direct measurement of cholesterol by cathodic stripping voltammetry is presented. The method is based on controlled adsorptive preconcentration at the static mercury drop electrode, followed by differential pulse voltammetric determination, in which adsorbed cholesterol reduces and shows stripping current at ca. −1.04 V (versus Ag/AgCl). The current was linear with the cholesterol concentration in the range of 0.2–8 μM. The detection limit found was 0.02 μM (10 minute preconcentration), and the relative standard deviation (at 0.6 μM level) 4.6%. Cyclic voltammetry was used to explore the adsorptive and electrochemical behavior. The adsorptive stripping response was evaluated with respect to preconcentration time, concentration dependence solution, stripping model, possible interference, and other variables. Applicability to serum analysis was illustrated.