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Electroanalytical determination of lormetazepam in pharmaceutical preparations and human urine
Author(s) -
Zapardiel A.,
Bermejo E.,
Pérez López J. A.,
Mateo P.,
Hernández L.
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040812
Subject(s) - adsorptive stripping voltammetry , hanging mercury drop electrode , polarography , chemistry , chromatography , relative standard deviation , mercury (programming language) , detection limit , dropping mercury electrode , urine , supporting electrolyte , pharmaceutical formulation , voltammetry , stripping (fiber) , differential pulse voltammetry , electrochemistry , electrode , cyclic voltammetry , materials science , inorganic chemistry , composite material , biochemistry , computer science , programming language
The electrochemical properties of the drug lormetazepam have been studied at the mercury electrode. A method for determination of the drug in commercial preparations by differential pulse polarography has been devised, with mean relative errors lower than 1.4% and relative standard deviations lower than 3.3%. Controlled adsorptive accumulation of lormetazepam at a hanging mercury drop electrode (HMDE) provided the basis for its determination by stripping voltammetry at nanomolar concentration levels. The adsorptive stripping response was evaluated with respect to experimental conditions and parameters such as electrolyte, pH, accumulation potential, accumulation, time and concentration. A drug determination method for use in urine with previous extraction (98% recovery) has been developed, with a detection limit of 23 ngm·1 −1 (40 second accumulation time) and a relative standard deviation around 6.8%.