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Sensitive yeast bioelectrode to L‐lactate
Author(s) -
Kulys Juozas,
Wang Lizhong,
Razumas Valdemaras
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040503
Subject(s) - ascorbic acid , yeast , chemistry , amperometry , chromatography , vitamin c , ethanol , michaelis–menten kinetics , biochemistry , enzyme , enzyme assay , food science , electrochemistry , electrode
Amperometric L‐lactate bioelectrodes based on carbon paste chemically modified with yeast Hansenula anomala and mediators are described. The bioelectrodes acted over a potential range at 0.05 – 0.3 V (vs SCE), the sensitivity was 14 – 116 μM −1 cm −2 and optimum pH was at 7.6 – 7.7. The apparent Michaelis constant for the calibration curves of bioelectrodes was different for mediators and varied in the range from 1.3 to 18.9 mM of L‐lactate. The bioelectrode was insensitive to glucose and ethanol. The bioelectrode's response to ascorbic acid was 1.4% at the normal physiological level of vitamin C and L‐lactate in blood. The bioelectrode was used for L‐lactate determination in serum, and remained stable for more than 2 months. The biocatalytic action of the cytochrome b 2 in yeast was suggested.