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Multienzyme containing tissue‐based and ferrocene‐mediated bioelectrode for the determination of polyamines
Author(s) -
Lin Meng Shan,
Hare Minoru,
Rechnitz Garry A.
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040502
Subject(s) - spermidine , spermine , polyamine oxidase , polyamine , chemistry , hydrogen peroxide , cadaverine , detection limit , biosensor , peroxidase , ferrocene , chromatography , biochemistry , nuclear chemistry , enzyme , electrode , electrochemistry
Oat seedling tissue and ferrocene/ferricinium were coimmobilized in a carbon paste matrix to construct a biosensor for polyamines. The oat tissue is rich in the enzymes polyamine oxidase and peroxidase while the mediator facilitates electron transfer between the electrode and hydrogen peroxide. The determination of the polyamines spermine and spermidine, in both batch and flow injection modes, was evaluated. The concentration of polyamine was monitored through hydrogen peroxide in the presence of oxygen as cosubstrate of polyamine oxidase. A reductive potential of 0.0 V was chosen for monitoring ferricinium which is generated by peroxidase. The detection limit (S/N = 3) for spermidine and spermine in the batch system was 0.19 and 1.15 μM, respectively. The relative standard deviation for twelve replicate analyses of 10 μM spermidine was 4.5%. The activity of the tissue modified carbon paste electrode decreased to 45% of the original value after a storage for 8 days in buffer solution at 4°C.