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Determination of 2‐thiothiazolidine‐4‐carboxylic acid in urine by liquid chromatography with electrochemical detection
Author(s) -
Ciucu Anton,
Baldwin Richard P.
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040420
Subject(s) - amperometry , detection limit , chromatography , urine sample , electrochemistry , chemistry , urine , glassy carbon , electrode , cobalt , working electrode , phthalocyanine , carboxylic acid , inorganic chemistry , organic chemistry , cyclic voltammetry , biochemistry
An assay procedure utilizing liquid chromatography and amperometric detection at a chemically modified electrode (CME) has been developed for the determination of 2‐thiothiazolidine‐4‐carboxylic acid (TTCA) in urine samples. The electrocatalytic activity of the CME, which consisted of a glassy carbon surface coated with an electropolymerized cobalt phthalocyanine film, permitted optimum response at a potential of +0.75 V vs. Ag/AgCl with a detection limit of 7 pmol for TTCA and with no sample preparation required. In case of fouling of the electrode surface by sample constituents, the polymeric cobalt phthalocyanine electrode surface could be renewed with a variability of less than 10% and an equilibration time of less than 15 minutes.