Premium
An electrochemical assay for tissue plasminogen activator (t‐PA)
Author(s) -
Cardosi Marco,
Ho Wah On,
Athey Dale,
McNeil Calum
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040305
Subject(s) - tripeptide , electrochemistry , detection limit , electrode , plasmin , chemistry , glassy carbon , cyclic voltammetry , t plasminogen activator , plasminogen activator , tissue plasminogen activator , chromatography , nuclear chemistry , enzyme , biochemistry , peptide , medicine
An electrochemical assay for tissue plasminogen activator (t‐PA) is described. The activity of t‐PA is measured in a coupled assay following the amidolytic cleavage of a synthetic tripeptide by the enzyme plasmin. The released electroactive label, p‐aminophenol, is detected anodically either at a glassy carbon indicator electrode or at a platinized activated carbon electrode in a specially constructed perspex cell. Calibration plot data for t‐PA are presented for buffered samples using the perspex block‐type cell. The limit of detection for t‐PA in this assay is 0.25 IU/ml. In addition, the assay format is adapted to yield kinetic information using repetitive square wave voltammetry as the mode of detection.