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Biosensor for detection of organophosphate and carbamate insecticides
Author(s) -
Marty JL,
Sode K.,
Karube I.
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040217
Subject(s) - paraoxon , choline oxidase , carbamate , organophosphate , acetylcholinesterase , chemistry , hydrogen peroxide , biosensor , chromatography , phosphate buffered saline , enzyme , pesticide , biochemistry , biology , agronomy
An enzyme sensor for the determination of organophosphate and carbamate insecticides is described. The mechanism is based on the coimmobilization and sequential enzymatic reactions of acetylcholinesterase and choline oxidase. The inhibition of acetylcholinesterase is measured as decreased formation of hydrogen peroxide (H 2 O 2 ) as monitored by a H 2 O 2 sensor. As low as 10 nM paraoxon can be determined with this technique. The long‐term stability of the sensor is two months, when stored at 4°C in phosphate buffer, and one year in a dry state.