Microarray electrodes as biosensors

Microdisk and microhole array electrodes were used to construct amperometric glucose biosensors based on the detection of hydrogen peroxide. All‐chemical methods such as platinization, glucose oxidase immobilization, and electropolymerization permit electrodes of any size, shape, or surface complexity to be used for biosensors. This also gives the biosensor surface a multifunctional character, where the platinization decreases the operating potential, the immobilized enzyme provides the specificity, and the electropolymerized film prevents interferences and fouling. All of these functions are accomplished in a layer that is about 10 nm thick. The microdisk and microhole biosensors were linear to about 3 mM and 4 mM glucose, respectively, and were accurate, as checked by standard reference serum.