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Tomato seed‐based amperometric sensor for the determination of alcohols
Author(s) -
Ozsoz Mehmet,
Wang Joseph
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030710
Subject(s) - alcohol dehydrogenase , amperometry , ethanol , chemistry , alcohol , substrate (aquarium) , reactivity (psychology) , cofactor , chromatography , allyl alcohol , organic chemistry , electrode , enzyme , catalysis , biology , electrochemistry , medicine , ecology , alternative medicine , pathology
The use of tomato seeds as a source for alcohol dehydrogenase (ADH) for the determination of alcohols is described. The unique reactivity pattern of the tomato ADH is coupled with the speed and sensitivity of the mixed tissue/carbon paste configuration. The effect of experimental variables, such as pH, cofactor concentration, applied potential, or paste composition, is explored. In accordance to the substrate specificity, the lowest detection limits (in the micromolar level) are obtained for allyl alcohol and ethanol.