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Enzymatic determination of ethanol by flow injection analysis using a Kel‐F wax carbon paste electrode
Author(s) -
Wangsa Julie,
Danielson Neil D.
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030706
Subject(s) - wax , detection limit , glassy carbon , ethanol , electrode , chemistry , chromatography , polymer , carbon fibers , flow injection analysis , wine , materials science , organic chemistry , cyclic voltammetry , electrochemistry , composite material , food science , composite number
Surface passivation during oxidation of NADH at a fluorocarbon polymer‐carbon paste electrode (CPE) was about 25% less than that of the glassy carbon or Nujol‐oil CPE. After repeated injections of 1.5 mM NADH for 12 h, the Kel‐F wax CPE maintained about 85% of its initial response. The determination of ethanol in wine samples using soluble alcohol dehydrogenase stabilized by polyethyleneglycol was demonstrated. Linearity of response extended from the detection limit of 0.44 mM to 33 mM.