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Cobalt‐porphyrin‐nafion film on carbon microarray electrode to monitor oxygen for enzyme analysis of glucose
Author(s) -
Dong Shaojun,
Kuwana Theodore
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030607
Subject(s) - glucose oxidase , nafion , glutaraldehyde , electrode , cobalt , chemistry , porphyrin , biosensor , cyclic voltammetry , catalysis , inorganic chemistry , clark electrode , nuclear chemistry , electrochemistry , chromatography , organic chemistry , biochemistry , electrolyte
A microcarbon array electrode was modified by the placement of a Nafion film containing cobalt tetra‐methylpyridyl phorphyrin on its surface. This electrode was applied to the analysis of solution glucose when it was further modified by the immobilization of glucose oxidase on the outermost surface of the Nafion by the cross‐linking of serum albumin with glutaraldehyde. The concomitant decrease in the concentration of oxygen, as it was consumed in the enzymatic reaction of glucose with glucose oxidase, was determined by either cyclic voltammetry or a double potential step method at the porphyrin‐Nafion catalytic electrode. Glucose could be determined in the range, of 0.01‐;4 mM rapidly, without interference from substances such as accurate or other saccharides.