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Study of the adsorptive stripping square‐wave voltammetry of bovine serum albumin
Author(s) -
Flores J. Rodríguez,
Marín C.,
Gonzalez E.,
Pariente F.,
Lorenzo E.
Publication year - 1991
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140030426
Subject(s) - square wave , bovine serum albumin , chromatography , chemistry , stripping (fiber) , calibration curve , analytical chemistry (journal) , electrolyte , serum albumin , adsorptive stripping voltammetry , voltammetry , detection limit , materials science , voltage , electrode , biochemistry , electrochemistry , physics , quantum mechanics , composite material
Abstract The adsorptive stripping square‐wave voltammetric behavior of bovine serum albumin (BSA) is described. Conditions were optimized for the determination of this protein with respect to accumulation potential, accumulation time, drop size, scan rate, frequency value, and electrolyte concentration. Under these optimum conditions for the determination of BSA, two calibration plots were obtained. For an accumulation time of 60 seconds, the plot was linear in the range 0.25–1.25 μg/mL of BSA ( r = 0.997); for an accumulation time of 180 seconds, the linear range was 0.10–1.25 μg/mL of BSA ( r = 0.997). The square‐wave procedure offers the advantages of speed, higher sensitivity for the determination of BSA, and the discrimination against background currents. This method was applied successfully for the determination of total protein in fetal calf serum.